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2002年10-11月,应用聚丙烯酰胺凝胶水平电泳,对我国新疆丁群体和从捷克引进的丁鱼岁群体各30尾个体的ADH、IDH、LDH、SDH、SOD、EST、MDH、ME、6PGDH、G3PDH等10种同工酶和1种肌蛋白进行分析。结果发现,我国丁鱼岁群体在EST-2、SOD、ADH和IDH等4种同工酶存在多态,捷克群体在EST-1、ADH和IDH等3种同工酶存在多态。我国新疆丁群体多态位点比例为28.57%,比捷克丁群体高(21.43%)。平均杂合度分别为0.061 9,也比捷克丁群体高(0.035 7)。捷克丁群体存在一定的杂合子缺失现象。因此,从遗传保护角度,应防止引入我国的捷克丁鱼岁群体进入新疆额尔齐斯河流域进行人工养殖,以免对我国丁天然资源造成污染。 相似文献
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This study tested KUROKURA solution (Kurokura et al., 1984, Aquaculture 37, 267–274) and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of potential motility of tench spermatozoa. The immobilizing solution is used because, when collected, the sperm of most samples is contaminated by urine, causing spermatozoa to be of poor quality, with low motilities and velocities (almost 0), thus resulting in a worsened fertilization and hatching rate. Sperm was sampled with a syringe containing an immobilizing solution (IS), allowing an IS:sperm ratio of 2:1, under aerobic conditions at 0–4°C. This sperm solution was stored for 10 h and untreated sperm was collected prior to fertilization as a control. Spermatozoa quality was evaluated for the cell motility and velocity parameters and also for fertilization ability and hatching rate. Results obtained for tench sperm motility, velocity, fertilization and hatching rate showed that only sperm collected in the various immobilizing solutions can be successfully used for artificial insemination and preservation after 10 h at 0–4°C. The best immobilizing solution was found to be KUROKURA 180 (180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2· 2H2O and 2.38 mM NaHCO3), giving a fertility and hatching rate of 41%, with no change in rates after 10 h storage of sperm. Control sperm without immobilizing solution showed a fertilization and hatching rate of only 6–7%. 相似文献
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Like marine fish freshwater fish are an important source of essential fatty acids for human nutrition. However, the fatty
acid composition of pond fish can vary considerably and strongly depends on that of the ingested food. Investigations on the
fatty acid composition of common carp (Cyprinus carpio) and tench (Tinca tinca) have shown that different methods of rearing and feeding cause substantial variations in the proportions of the n-6 and
n-3 polyunsaturated fatty acids of these fish species. Carp reared on the basis of natural food in ponds exhibit high contents
of n-6 as well as n-3 fatty acids in their muscle triacylglycerols. On the other hand carp fed supplementary wheat in ponds
resulted in somewhat lower levels of these essential fatty acids. High amounts of n-3 fatty acids can be found in carp fed
high-energy diets containing high levels of fish oil. Analogous results were obtained in experiments with tench reared under
different nutritional conditions. While rearing on the basis of only natural food in ponds as well as feeding supplementary
wheat yielded in similar levels of n-3 and n-6 polyunsaturated fatty acids, higher contents of n-3 fatty acids were recorded
in tench fed pellets. High levels of n-3 polyunsaturated fatty acids in foodstuffs have positive effects on human health.
Experiments with different cultured fish species proved that the fatty acid composition of the edible parts can be influenced
by the diet. Therefore, a finishing diet with a suitable fatty acid profile can be used to improve the nutritional quality
of fish products of farmed origin. 相似文献
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对丁(Tincatinca)血液有形成分及血清无机盐进行了测定,实验结果显示:①血液有形成分:丁的红细胞计数为1.43×1012～5.90×1012/L,平均为3.11×1012/L;红细胞平均体积为67.52fL,红细胞压积平均为0.11%;白细胞计数为9.32×109～54.10×109/L,平均为33.84×109/L,其中白细胞淋巴球占白细胞的56.28%,中性白细胞占43.72%;血小板数量为103.72×109/L,血红素平均含量为121.18g/L。丁的红细胞具有体积小,数量多的特点,其血液可以携带更多氧分而能忍受水中较低的溶氧。血小板和白细胞淋巴球的含量远远高于本地鲤鱼或鲫鱼,这有利于丁的生长与抗病。②血清无机盐:丁血液平均含PO3-43.31mmol/L,K+11.11mmol/L,Na+125.23mmol/L,Cl-105.08mmol/L,Ca2+2.51mmol/L,Fe3+15.13mmol/L,Mg2+0.33mmol/L,CO(NH2)21.475mmol/L,其中K+和PO3-4的含量远远高出人的正常值。 相似文献
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Vanesa García José M. Carral María Sáez-Royuela Rocío González Álvaro González 《Journal Of Applied Aquaculture》2013,25(1):57-65
There are no specifically formulated dry foods for tench (Tinca tinca L.), which forces farmers to use diets formulated for other fish species. This has major drawbacks, such as high mortality, slow growth, and body deformities. A 120 day experiment was performed with five-month-old juvenile tench (initial mean weight: 0.388 g; total length: 31.78 mm) to evaluate decapsulated Artemia cysts as a supplement to a dry diet for other fish species. Three treatments, differing in the daily supplement, were tested: 1,800 freshly hatched nauplii, 1,800 cysts, and 300 cysts per g of tench biomass. Final survival ranged between 95.3% and 97.9%. Juvenile tench that received the supplement of 1,800 decapsulated Artemia cysts had a specific growth rate (1.28), weight (1.83 g), and total length (52.30 mm) significantly higher than those with the same amount of nauplii. The lowest supplement (300 cysts/g of fish biomass) allowed significantly lower growth and higher condition coefficient (1.40) than the rest. Animals with body deformities (1.06%) were only recorded in the groups that received the lowest cyst supplement. Results showed Artemia cysts are a suitable dietary supplement for juvenile tench, being an advantageous alternative to live nauplii. 相似文献
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【目的】研究丁鱥鱼在南方较高水温条件下的生长发育情况,解决丁鱥鱼在南方推广养殖中苗种欠缺的瓶颈问题。【方法】对丁鱥亲鱼进行人工繁殖,然后对其胚胎及仔鱼发育过程进行观察记录。【结果】丁鱥鱼催产效应时间11h,受精率为75.3%,孵化率为82.7%,仔鱼成活率59.3%,育成规格为1.5～2.0cm鱼苗20.0万尾。丁鱥鱼受精卵呈卵圆形,大部分呈青灰色,直径0.50～0.77mm,具粘性;在27.0～31.0℃的温度下,受精卵历时1936min发育后孵出,有效积温为908.89℃·h;胚胎发育可划分为6个阶段30个发育时期。刚孵出仔鱼全长3.84～3.87mm,出膜后第3d卵黄囊吸收60%~80%,约有30%仔鱼开口摄食;第4d卵黄囊吸收90%,约有90%仔鱼开口;出膜第5d卵黄囊基本吸收完,但出现少许死鱼苗。【结论】丁鱥鱼适宜在南方进行繁殖及鱼苗培育,水温应稳定在20.0～27.0℃,不宜超过30.0℃,在孵化及鱼苗培育过程中注意清除敌害生物及保持清新水质,并根据水温情况及时投喂开口饵料,提高仔鱼成活率。 相似文献
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【目的】探讨丁鱥鱼人工繁殖及鱼苗培育技术,满足广大水产养殖户对丁鱥鱼苗的需求,为加快丁鱥鱼在广西的推广养殖奠定基础。【方法】2004年11月和2005年3月,两次引进丁鱥1龄鱼进行培育,2005年春挑选成熟鱼进行人工繁育。【结果】亲鱼经过半年强化培育,平均体重600g/尾以上,雌雄鱼性腺发育到第Ⅳ-Ⅴ期;以促黄体生成素释放激素类似物（LRH—a2）和地欧酮（DOM）混合物催产,经11～13h的发情效应,均出现产卵行为;经人工孵化和鱼苗培育,第1批的人工孵化率最高,为85．0％,但由于采用池塘孵化,仔鱼遭受池塘敌害生物残食,培育失败;第2、3批的人工孵化率分别为59．3％和63．0％,采用小水泥池孵化,两批共孵化出仔鱼约93．9万尾,培育获得规格为3．0—5．0cm鱼苗64．8万尾。【结论】通过科学合理的亲鱼强化培育、药物催产、人工孵化及鱼苗培育,可以成功繁育获得丁鱥鱼苗,但繁育过程中要注意水温调控,当水温上升到20℃以上、最好稳定在23～27℃时,应及时安排生产,以利于提高丁鱥鱼苗数量和质量。 相似文献
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丁鱼岁“腐皮病”病原菌的分离鉴定与药敏试验 总被引：1，自引：1，他引：0
为了确定丁鱼岁腐皮病的病原菌,为该病的诊断和防治提供科学依据。采用细菌常规分离方法,从丁鱼岁体表病灶部位、内脏及血液分离出优势菌株GXZ01,对其进行了生理生化特性、人工感染试验及药敏试验研究。结果表明：（1）菌株GXZ01是温和气单胞菌。（2）该菌呈短杆状,长度约为0.7~1.1 μm,属革兰氏阴性菌;（3）该菌对氧哌嗪青霉素、氟哌酸、菌必治等8种抗生素敏感,对丙氟哌酸、氨苄青霉素等4种药物中度敏感,对青霉素和乙酰螺旋霉素不敏感。表明温和气单胞菌是致病菌,可以导致丁鱼岁腐皮病的发生,这尚属首次报道,将对该鱼的病害防治和健康养殖有着重要的指导意义。 相似文献
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Evaluation of pea protein concentrate as partial replacement of fish meal in practical diets for juvenile tench (Tinca tinca L.) 
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下载免费PDF全文 Álvaro González‐Rodríguez Jesús D Celada José M Carral María Sáez‐Royuela Juan B Fuertes 《Aquaculture Research》2016,47(9):2825-2834
With the aim to evaluate different replacement levels of fish meal (FM) by pea protein concentrate (PPC) on survival, growth performance and body composition of juvenile tench (Tinca tinca), a 90‐day experiment was conducted with 6‐month‐old juveniles. Four practical diets (50% crude protein) differing in the level of replacement of FM protein by PPC protein were tested: 0% (control), 25%, 35% or 45%, corresponding to 0, 207.5, 290.4 or 373.3 g PPC kg?1 diet respectively. Survival rates ranged from 96.4% to 98.5%. The 25% and 35% replacement diets resulted in similar growth values (P > 0.05) to those obtained with the control diet (average of the three feeding treatments: 57.57 mm total length, 2.48 g weight and 1.87% day?1 specific growth rate). The 45% replacement diet had the lowest growth (P < 0.05). Fish with externally visible deformities ranged from 0% to 1.5%. The relation among amino acid profiles of the diets, body composition, growth performance of juveniles and amino acid requirements of other fish species is discussed. An amount of 290.4 g PPC kg?1 diet (35% replacement of FM protein) can be included in juvenile tench diets without impairing growth performance. 相似文献